Frange P., Galimand J., Vidal Nicole, Goujard C., Deveau C., Souala F., Peeters Martine, Meyer L., Rouzioux C., Chaix M. L. (2008). New and old complex recombinant HIV-1 strains among patients with primary infection in 1996-2006 in France : the French ANRS CO06 primo cohort study - art. no. 69. Retrovirology, 5 (69), p. 1. ISSN 1742-4690.
Titre du document
New and old complex recombinant HIV-1 strains among patients with primary infection in 1996-2006 in France : the French ANRS CO06 primo cohort study - art. no. 69
Année de publication
2008
Auteurs
Frange P., Galimand J., Vidal Nicole, Goujard C., Deveau C., Souala F., Peeters Martine, Meyer L., Rouzioux C., Chaix M. L.
Source
Retrovirology, 2008,
5 (69), p. 1 ISSN 1742-4690
Background: Prevalence of HIV-1 non-B subtypes has increased overtime in patients diagnosed at the time of primary infection (PHI) in France. Our objective was to characterize in detail non-B strains which could not be genetically classified into the known subtypes/Circulating Recombinant Forms (CRFs). Methods: Among 744 patients enrolled in the ANRS PRIMO Cohort since 1996, 176 (23.7%) were infected with HIV-1 non-B strains. The subtype/CRF could not be identified in RT for 15 (2%). The V3-V5 env region was sequenced and 3 strains (04FR-KZS, 06FR-CRN, 04FR-AUK) were full-length sequenced. Phylogenetic and bootscan analyses were used to characterize the mosaic structures. Results: Among V3-V5 sequences, 6 were divergent A, 2 distantly related to E or D, 2 C, 1 B and 2 remained unclassified. 04FR-KZS, isolated in a Congolese woman infected in France, clustered with 2 previously described viruses from the Democratic Republic of Congo. They represent CRF27_ cpx involving A/E/G/H/J/K/U subtypes. 06FR-CRN, isolated in a homosexual Caucasian patient, was a B/C/U recombinant involving a Brazilian C strain. 04FR-AUK, isolated in a Congolese patient infected in France, was a A/K/CRF09/U recombinant clustering from gag to vif with HIV-1 MAL. Others PHI were further observed in 2006 -2007 with 1 KZS and 5 CRN-like viruses, suggesting their spread in France. Conclusion: This study illustrates the increasing HIV-1 diversity in France associating new (06FR-CRN) and old (CRF27_cpx and "MAL-like" 04FR-AUK) strains, which are rare in their region of origin but may have a possible founder effect in France. Our results strengthen the French guidelines recommending viro-epidemiological surveillance of HIV-1 diversity.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010044037]
Identifiant IRD
fdi:010044037
