%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Frange, P.
%A Galimand, J.
%A Vidal, Nicole
%A Goujard, C.
%A Deveau, C.
%A Souala, F.
%A Peeters, Martine
%A Meyer, L.
%A Rouzioux, C.
%A Chaix, M. L.
%T New and old complex recombinant HIV-1 strains among patients with primary infection in 1996-2006 in France : the French ANRS CO06 primo cohort study - art. no. 69
%D 2008
%L fdi:010044037
%G ENG
%J Retrovirology
%@ 1742-4690
%M ISI:000259484800001
%N 69
%P 1
%R 10.1186/1742-4690-5-69
%U https://www.documentation.ird.fr/hor/fdi:010044037
%> https://www.documentation.ird.fr/intranet/publi/2008/10/010042813.pdf
%V 5
%W Horizon (IRD)
%X Background: Prevalence of HIV-1 non-B subtypes has increased overtime in patients diagnosed at the time of primary infection (PHI) in France. Our objective was to characterize in detail non-B strains which could not be genetically classified into the known subtypes/Circulating Recombinant Forms (CRFs). Methods: Among 744 patients enrolled in the ANRS PRIMO Cohort since 1996, 176 (23.7%) were infected with HIV-1 non-B strains. The subtype/CRF could not be identified in RT for 15 (2%). The V3-V5 env region was sequenced and 3 strains (04FR-KZS, 06FR-CRN, 04FR-AUK) were full-length sequenced. Phylogenetic and bootscan analyses were used to characterize the mosaic structures. Results: Among V3-V5 sequences, 6 were divergent A, 2 distantly related to E or D, 2 C, 1 B and 2 remained unclassified. 04FR-KZS, isolated in a Congolese woman infected in France, clustered with 2 previously described viruses from the Democratic Republic of Congo. They represent CRF27_ cpx involving A/E/G/H/J/K/U subtypes. 06FR-CRN, isolated in a homosexual Caucasian patient, was a B/C/U recombinant involving a Brazilian C strain. 04FR-AUK, isolated in a Congolese patient infected in France, was a A/K/CRF09/U recombinant clustering from gag to vif with HIV-1 MAL. Others PHI were further observed in 2006 -2007 with 1 KZS and 5 CRN-like viruses, suggesting their spread in France. Conclusion: This study illustrates the increasing HIV-1 diversity in France associating new (06FR-CRN) and old (CRF27_cpx and "MAL-like" 04FR-AUK) strains, which are rare in their region of origin but may have a possible founder effect in France. Our results strengthen the French guidelines recommending viro-epidemiological surveillance of HIV-1 diversity.
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