Flachsland E., Terada G., Scocchi A., Rey H., Mroginski L., Engelmann Florent. (2006). Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium Sims. (Orchidaceae) by encapsulation-dehydration. Cryoletters, 27 (4), p. 235-242. ISSN 0143-2044.
Titre du document
Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium Sims. (Orchidaceae) by encapsulation-dehydration
Flachsland E., Terada G., Scocchi A., Rey H., Mroginski L., Engelmann Florent
Source
Cryoletters, 2006,
27 (4), p. 235-242 ISSN 0143-2044
Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing 1/2 MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for I h, thawed at 30 degrees C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on 1/2 MS medium with 0.1 M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
