@article{fdi:010037804,
  title = {{C}ryopreservation of seeds and in vitro-cultured protocorms of {O}ncidium bifolium {S}ims. ({O}rchidaceae) by encapsulation-dehydration},
  author = {{F}lachsland, {E}. and {T}erada, {G}. and {S}cocchi, {A}. and {R}ey, {H}. and {M}roginski, {L}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{E}ncapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of {O}ncidium bifolium. {F}reshly harvested seeds, 120 days after pollination, were encapsulated in beads containing 1/2 {MS} medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 {M} sucrose (24 h) followed by 0.25 {M} sucrose (48 h), 0.5 {M} sucrose (24 h) and 0.75 {M} sucrose (24 h). {T}he beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for {I} h, thawed at 30 degrees {C} for 2 min, post-treated using the same series of liquid media [0.5 {M} sucrose (24 h), 0.25 {M} sucrose (48 h), 0.15 {M} sucrose (24 h)], and recultured on 1/2 {MS} medium with 0.1 {M} sucrose and 0.7% agar. {A}s much as 4.8% of the cryopreserved seeds produced complete plants. {I}n-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.},
  keywords = {{O}ncidium bifolium ; {O}rchidaceae ; cryopreservation ; encapsulation dehydration ; seeds ; protocorms},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {27},
  numero = {4},
  pages = {235--242},
  ISSN = {0143-2044},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037804},
}