%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Flachsland, E.
%A Terada, G.
%A Scocchi, A.
%A Rey, H.
%A Mroginski, L.
%A Engelmann, Florent
%T Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium Sims. (Orchidaceae) by encapsulation-dehydration
%D 2006
%L fdi:010037804
%G ENG
%J Cryoletters
%@ 0143-2044
%K Oncidium bifolium ; Orchidaceae ; cryopreservation ; encapsulation dehydration ; seeds ; protocorms
%M CC:0002409037-0004
%N 4
%P 235-242
%U https://www.documentation.ird.fr/hor/fdi:010037804
%> https://www.documentation.ird.fr/intranet/publi/2007/suppl/010037804.pdf
%V 27
%W Horizon (IRD)
%X Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing 1/2 MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for I h, thawed at 30 degrees C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on 1/2 MS medium with 0.1 M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
%$ 076