Kim H. H., Yoon J. W., Park Y. E., Cho E. G., Sohn J. K., Kim T. S., Engelmann Florent. (2006). Cryopreservation of potato cultivated varieties and wild species : critical factors in droplet vitrification. Cryoletters, 27 (4), p. 223-234. ISSN 0143-2044.
Titre du document
Cryopreservation of potato cultivated varieties and wild species : critical factors in droplet vitrification
Kim H. H., Yoon J. W., Park Y. E., Cho E. G., Sohn J. K., Kim T. S., Engelmann Florent
Source
Cryoletters, 2006,
27 (4), p. 223-234 ISSN 0143-2044
The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40 degrees C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.
