@article{fdi:010037799,
  title = {{C}ryopreservation of potato cultivated varieties and wild species : critical factors in droplet vitrification},
  author = {{K}im, {H}. {H}. and {Y}oon, {J}. {W}. and {P}ark, {Y}. {E}. and {C}ho, {E}. {G}. and {S}ohn, {J}. {K}. and {K}im, {T}. {S}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. {W}e tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. {T}he droplet-vitrification method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. {H}igh survival after freezing was observed after dehydration with {PVS}2 for 20 min, cooling shoot tips placed in a droplet of {PVS}2 solution on aluminum foil strips by immersing the foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 {M} sucrose solution (at 40 degrees {C}) for 30 s and unloading in 0.8 {M} sucrose for 30 min. {T}his optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.},
  keywords = {droplet vitrification ; {PVS}2/{PVS}3 loading ; shoot tips ; {S}olanum tuberosum {L}.},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {27},
  numero = {4},
  pages = {223--234},
  ISSN = {0143-2044},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037799},
}