%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Kim, H. H.
%A Yoon, J. W.
%A Park, Y. E.
%A Cho, E. G.
%A Sohn, J. K.
%A Kim, T. S.
%A Engelmann, Florent
%T Cryopreservation of potato cultivated varieties and wild species : critical factors in droplet vitrification
%D 2006
%L fdi:010037799
%G ENG
%J Cryoletters
%@ 0143-2044
%K droplet vitrification ; PVS2/PVS3 loading ; shoot tips ; Solanum tuberosum L.
%M CC:0002409037-0003
%N 4
%P 223-234
%U https://www.documentation.ird.fr/hor/fdi:010037799
%> https://www.documentation.ird.fr/intranet/publi/2007/suppl/010037799.pdf
%V 27
%W Horizon (IRD)
%X The applicability of cryopreservation protocols to a broad range of genotypes is a key issue for genebanks. We tried to identify the critical factors causing differences in survival of cryopreserved shoot tips using potato varieties coming from cultivated and wild species. The droplet-vitrification method, a combination of droplet-freezing and solution-based vitrification, was selected from several protocols. High survival after freezing was observed after dehydration with PVS2 for 20 min, cooling shoot tips placed in a droplet of PVS2 solution on aluminum foil strips by immersing the foil strips in liquid nitrogen, warming them by plunging the foil strips into a 0.8 M sucrose solution (at 40 degrees C) for 30 s and unloading in 0.8 M sucrose for 30 min. This optimized protocol was successfully applied to 12 accessions with survival ranging between 64.0 and 94.4%.
%$ 076