Molouba F., Lorquin Jean, Willems A., Hoste B., Giraud Eric, Dreyfus Bernard, Gillis M., Lajudie Philippe de, Masson Boivin Catherine. (1999). Photosynthetic bradyrhizobia from Aeschynomene spp are specific to stem-nodulated species and form a separate 16S ribosomal DNA restriction fragment length polymorphism group. Applied and Environmental Microbiology, 65 (7), p. 3084-3094. ISSN 0099-2240.
Titre du document
Photosynthetic bradyrhizobia from Aeschynomene spp are specific to stem-nodulated species and form a separate 16S ribosomal DNA restriction fragment length polymorphism group
Année de publication
1999
Auteurs
Molouba F., Lorquin Jean, Willems A., Hoste B., Giraud Eric, Dreyfus Bernard, Gillis M., Lajudie Philippe de, Masson Boivin Catherine
Source
Applied and Environmental Microbiology, 1999,
65 (7), p. 3084-3094 ISSN 0099-2240
We obtained nine bacterial isolates from root or collar nodules of the non-stem-nodulated Aeschynomene species A. elaphroxylon, A. uniflora, or A. schimperi and 69 root or stem nodule isolates from the stem-nodulated Aeschynomene species A. afraspera, A. ciliata, A. indica, A. nilotica, A. sensitiva, and A. tambacoundensis from various places in Senegal. These isolates, together with 45 previous isolates from various Aeschynomene species, were studied for host-specific nodulation within the genus Aeschynomene, also revisiting cross-inoculation groups described previously by D. Alazard (Appl. Environ. Microbiol. 50:732-734, 1985). The whole collection of Aeschynomene nodule isolates was screened for synthesis of photosynthetic pigments by spectrometry, high pressure liquid chromatography, and thin layer chromatography analyses. The presence of puf genes in photosynthetic Aeschynomene isolates was evidenced both by Southern hybridization with a Rhodobacter capsulatus photosynthetic gene probe and by DNA amplificaton with primers defined from photosynthetic genes. In addition, amplified 16S ribosomal DNA restriction analysis was performed on 45 Aeschynomene isolates, including strain BTAi1, and 19 reference strains from Bradyrhizobium japonicum, Bradyrhizobium elkani, and other Bradyrhizobium sp. strains of uncertain taxonomic positions. The 16S rRNA gene sequence of the photosynthetic strain ORS278 (LMG 12187) was determined and compared to sequences from databases. Our main conclusion is that photosynthetic Aeschynomene nodule isolates share the ability to nodulate particular stem-modulated species and form a separate subbranch on the Bradyrhizobium rRNA, distinct from B. japonicum and B. elkanii. (Résumé d'auteur)
Plan de classement
Fixation biologique de l'azote [084SYMBIO02]
Descripteurs
LEGUMINEUSE TROPICALE ; BACTERIE ; NODULE RACINAIRE ; FIXATION BIOLOGIQUE DE L'AZOTE ; PIGMENT ; PHOTOSYNTHESE ; HYBRIDATION ; TECHNIQUE PCR ; TECHNIQUE RFLP ; SPECTROMETRIE ; CHROMATOGRAPHIE EN PHASE LIQUIDE ; ETUDE EXPERIMENTALE ; ETUDE COMPARATIVE ; SOUCHE BTAI1 ; GENE PUF
Description Géographique
SENEGAL
Localisation
Fonds IRD [F B010023993] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010023993
