%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Molouba, F.
%A Lorquin, Jean
%A Willems, A.
%A Hoste, B.
%A Giraud, Eric
%A Dreyfus, Bernard
%A Gillis, M.
%A Lajudie, Philippe de
%A Masson Boivin, Catherine
%T Photosynthetic bradyrhizobia from Aeschynomene spp are specific to stem-nodulated species and form a separate 16S ribosomal DNA restriction fragment length polymorphism group
%D 1999
%L fdi:010023993
%G ENG
%J Applied and Environmental Microbiology
%@ 0099-2240
%K LEGUMINEUSE TROPICALE ; BACTERIE ; NODULE RACINAIRE ; FIXATION BIOLOGIQUE DE L'AZOTE ; PIGMENT ; PHOTOSYNTHESE ; HYBRIDATION ; TECHNIQUE PCR ; TECHNIQUE RFLP ; SPECTROMETRIE ; CHROMATOGRAPHIE EN PHASE LIQUIDE ; ETUDE EXPERIMENTALE ; ETUDE COMPARATIVE
%K SOUCHE BTAI1 ; GENE PUF
%K SENEGAL
%N 7
%P 3084-3094
%U https://www.documentation.ird.fr/hor/fdi:010023993
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_57-58/010023993.pdf
%V 65
%W Horizon (IRD)
%X We obtained nine bacterial isolates from root or collar nodules of the non-stem-nodulated #Aeschynomene$ species #A. elaphroxylon$, #A. uniflora$, or #A. schimperi$ and 69 root or stem nodule isolates from the stem-nodulated #Aeschynomene$ species #A. afraspera$, #A. ciliata$, #A. indica$, #A. nilotica$, #A. sensitiva$, and #A. tambacoundensis$ from various places in Senegal. These isolates, together with 45 previous isolates from various #Aeschynomene$ species, were studied for host-specific nodulation within the genus #Aeschynomene$, also revisiting cross-inoculation groups described previously by D. Alazard (Appl. Environ. Microbiol. 50:732-734, 1985). The whole collection of #Aeschynomene$ nodule isolates was screened for synthesis of photosynthetic pigments by spectrometry, high pressure liquid chromatography, and thin layer chromatography analyses. The presence of puf genes in photosynthetic #Aeschynomene$ isolates was evidenced both by Southern hybridization with a #Rhodobacter capsulatus$ photosynthetic gene probe and by DNA amplificaton with primers defined from photosynthetic genes. In addition, amplified 16S ribosomal DNA restriction analysis was performed on 45 #Aeschynomene$ isolates, including strain BTAi1, and 19 reference strains from #Bradyrhizobium japonicum$, #Bradyrhizobium elkani$, and other #Bradyrhizobium$ sp. strains of uncertain taxonomic positions. The 16S rRNA gene sequence of the photosynthetic strain ORS278 (LMG 12187) was determined and compared to sequences from databases. Our main conclusion is that photosynthetic #Aeschynomene$ nodule isolates share the ability to nodulate particular stem-modulated species and form a separate subbranch on the #Bradyrhizobium$ rRNA, distinct from #B. japonicum$ and #B. elkanii$. (Résumé d'auteur)
%$ 084SYMBIO02