@article{fdi:010023993,
  title = {{P}hotosynthetic bradyrhizobia from {A}eschynomene spp are specific to stem-nodulated species and form a separate 16{S} ribosomal {DNA} restriction fragment length polymorphism group},
  author = {{M}olouba, {F}. and {L}orquin, {J}ean and {W}illems, {A}. and {H}oste, {B}. and {G}iraud, {E}ric and {D}reyfus, {B}ernard and {G}illis, {M}. and {L}ajudie, {P}hilippe de and {M}asson {B}oivin, {C}atherine},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e obtained nine bacterial isolates from root or collar nodules of the non-stem-nodulated #{A}eschynomene$ species #{A}. elaphroxylon$, #{A}. uniflora$, or #{A}. schimperi$ and 69 root or stem nodule isolates from the stem-nodulated #{A}eschynomene$ species #{A}. afraspera$, #{A}. ciliata$, #{A}. indica$, #{A}. nilotica$, #{A}. sensitiva$, and #{A}. tambacoundensis$ from various places in {S}enegal. {T}hese isolates, together with 45 previous isolates from various #{A}eschynomene$ species, were studied for host-specific nodulation within the genus #{A}eschynomene$, also revisiting cross-inoculation groups described previously by {D}. {A}lazard ({A}ppl. {E}nviron. {M}icrobiol. 50:732-734, 1985). {T}he whole collection of #{A}eschynomene$ nodule isolates was screened for synthesis of photosynthetic pigments by spectrometry, high pressure liquid chromatography, and thin layer chromatography analyses. {T}he presence of puf genes in photosynthetic #{A}eschynomene$ isolates was evidenced both by {S}outhern hybridization with a #{R}hodobacter capsulatus$ photosynthetic gene probe and by {DNA} amplificaton with primers defined from photosynthetic genes. {I}n addition, amplified 16{S} ribosomal {DNA} restriction analysis was performed on 45 #{A}eschynomene$ isolates, including strain {BTA}i1, and 19 reference strains from #{B}radyrhizobium japonicum$, #{B}radyrhizobium elkani$, and other #{B}radyrhizobium$ sp. strains of uncertain taxonomic positions. {T}he 16{S} r{RNA} gene sequence of the photosynthetic strain {ORS}278 ({LMG} 12187) was determined and compared to sequences from databases. {O}ur main conclusion is that photosynthetic #{A}eschynomene$ nodule isolates share the ability to nodulate particular stem-modulated species and form a separate subbranch on the #{B}radyrhizobium$ r{RNA}, distinct from #{B}. japonicum$ and #{B}. elkanii$. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{LEGUMINEUSE} {TROPICALE} ; {BACTERIE} ; {NODULE} {RACINAIRE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {PIGMENT} ; {PHOTOSYNTHESE} ; {HYBRIDATION} ; {TECHNIQUE} {PCR} ; {TECHNIQUE} {RFLP} ; {SPECTROMETRIE} ; {CHROMATOGRAPHIE} {EN} {PHASE} {LIQUIDE} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {SOUCHE} {BTAI}1 ; {GENE} {PUF} ; {SENEGAL}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {65},
  numero = {7},
  pages = {3084--3094},
  ISSN = {0099-2240},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010023993},
}