Gonzalez-Arnao M.T., Urra C., Engelmann Florent, Ortiz R., Fe C. de la. (1999). Cryopreservation of encapsulated sugarcane apices : effect of storage temperature and storage duration. Cryo-Letters, 20 (6), p. 347-352.
Titre du document
Cryopreservation of encapsulated sugarcane apices : effect of storage temperature and storage duration
Année de publication
1999
Type de document
Article
Auteurs
Gonzalez-Arnao M.T., Urra C., Engelmann Florent, Ortiz R., Fe C. de la
Source
Cryo-Letters, 1999,
20 (6), p. 347-352
Apices sampled on in vitro plantlets of sugarcane were cryopreserved using the encapsulation-dehydration technique, comprising pregrowth for 24 h in liquid medium with 0.75 M sucrose, dessication to 20-25% moisture content (fresh weight basis) and rapid immersion in liquid nitrogen. Apices of one variety were stored for up to one year at -196°C, -70°C or -25°C, with or without previous transitory immersion in liquid nitrogen. Whereas the recovery percentage of apices stored at -196°C remained high and constant throughout the experiment, recovery of apices conserved at -70°C and -25°C decreased dramatically within 0.5 day, and was nil after 10 or 120 days in storage at -25°C and -70°C, respectively. The recovery percentage of apices of four additional varieties remained stable throughout a 1-year storage period at -196°C. No differences were noted for 6 agronomic traits between plants regenerated from control and cryopreserved apices of two sugarcane varieties. (Résumé d'auteur)
Plan de classement
Collection, conservation [076AMEPLA02]
;
Culture végétale in vitro [084VITRO]
Descripteurs
CANNE A SUCRE ; CRYOCONSERVATION ; CONSERVATION DES RESSOURCES GENETIQUES ; TECHNIQUE ; AZOTE ; DESHYDRATATION ; TEMPERATURE ; DUREE ; STOCKAGE ; ETUDE EXPERIMENTALE ; ENCAPSULATION ; APEX
