@article{fdi:010019815,
  title = {{C}ryopreservation of encapsulated sugarcane apices : effect of storage temperature and storage duration},
  author = {{G}onzalez-{A}rnao, {M}.{T}. and {U}rra, {C}. and {E}ngelmann, {F}lorent and {O}rtiz, {R}. and {F}e, {C}. de la},
  editor = {},
  language = {{ENG}},
  abstract = {{A}pices sampled on in vitro plantlets of sugarcane were cryopreserved using the encapsulation-dehydration technique, comprising pregrowth for 24 h in liquid medium with 0.75 {M} sucrose, dessication to 20-25% moisture content (fresh weight basis) and rapid immersion in liquid nitrogen. {A}pices of one variety were stored for up to one year at -196°{C}, -70°{C} or -25°{C}, with or without previous transitory immersion in liquid nitrogen. {W}hereas the recovery percentage of apices stored at -196°{C} remained high and constant throughout the experiment, recovery of apices conserved at -70°{C} and -25°{C} decreased dramatically within 0.5 day, and was nil after 10 or 120 days in storage at -25°{C} and -70°{C}, respectively. {T}he recovery percentage of apices of four additional varieties remained stable throughout a 1-year storage period at -196°{C}. {N}o differences were noted for 6 agronomic traits between plants regenerated from control and cryopreserved apices of two sugarcane varieties. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CANNE} {A} {SUCRE} ; {CRYOCONSERVATION} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {TECHNIQUE} ; {AZOTE} ; {DESHYDRATATION} ; {TEMPERATURE} ; {DUREE} ; {STOCKAGE} ; {ETUDE} {EXPERIMENTALE} ; {ENCAPSULATION} ; {APEX}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {20},
  numero = {6},
  pages = {347--352},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010019815},
}