%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Gonzalez-Arnao, M.T.
%A Urra, C.
%A Engelmann, Florent
%A Ortiz, R.
%A Fe, C. de la
%T Cryopreservation of encapsulated sugarcane apices : effect of storage temperature and storage duration
%D 1999
%L fdi:010019815
%G ENG
%J Cryo-Letters
%K CANNE A SUCRE ; CRYOCONSERVATION ; CONSERVATION DES RESSOURCES GENETIQUES ; TECHNIQUE ; AZOTE ; DESHYDRATATION ; TEMPERATURE ; DUREE ; STOCKAGE ; ETUDE EXPERIMENTALE
%K ENCAPSULATION ; APEX
%N 6
%P 347-352
%U https://www.documentation.ird.fr/hor/fdi:010019815
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_51-52/010019815.pdf
%V 20
%W Horizon (IRD)
%X Apices sampled on in vitro plantlets of sugarcane were cryopreserved using the encapsulation-dehydration technique, comprising pregrowth for 24 h in liquid medium with 0.75 M sucrose, dessication to 20-25% moisture content (fresh weight basis) and rapid immersion in liquid nitrogen. Apices of one variety were stored for up to one year at -196°C, -70°C or -25°C, with or without previous transitory immersion in liquid nitrogen. Whereas the recovery percentage of apices stored at -196°C remained high and constant throughout the experiment, recovery of apices conserved at -70°C and -25°C decreased dramatically within 0.5 day, and was nil after 10 or 120 days in storage at -25°C and -70°C, respectively. The recovery percentage of apices of four additional varieties remained stable throughout a 1-year storage period at -196°C. No differences were noted for 6 agronomic traits between plants regenerated from control and cryopreserved apices of two sugarcane varieties. (Résumé d'auteur)
%$ 076AMEPLA02 ; 084VITRO