Wu Y., Engelmann Florent, Zhao Y., Zhou M., Chen S. (1999). Cryopreservation of apple shoot tips : importance of cryopreservation technique and of conditioning of donor plants. Cryo-Letters, 20 (2), p. 121-130. ISSN 0143-2044.
Titre du document
Cryopreservation of apple shoot tips : importance of cryopreservation technique and of conditioning of donor plants
Année de publication
1999
Type de document
Article
Auteurs
Wu Y., Engelmann Florent, Zhao Y., Zhou M., Chen S.
Source
Cryo-Letters, 1999,
20 (2), p. 121-130 ISSN 0143-2044
In this paper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing in vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. Cold-hardening mother plants for 3 weeks at 5°C improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. Increasing the time during which mother-plants were maintained on standard medium without subculture before sampling of apices decreased the water content of shoot tips from about 85-88% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulation-dehydration technique. Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apcies, thus simplyfying the protocol. This simplified encapsulation-dehydration protocol was successfully applied to 11 out of the 12 cultivars tested. (Résumé d'auteur)
Plan de classement
Collection, conservation [076AMEPLA02]
;
Culture végétale in vitro [084VITRO]
