%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Wu, Y.
%A Engelmann, Florent
%A Zhao, Y.
%A Zhou, M.
%A Chen, S.
%T Cryopreservation of apple shoot tips : importance of cryopreservation technique and of conditioning of donor plants
%D 1999
%L fdi:010018692
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K CULTURE IN VITRO ; CRYOCONSERVATION ; CONDITIONNEMENT ; PLANTE ; REGENERATION ; METHODOLOGIE ; ETUDE COMPARATIVE ; MATERIEL VEGETAL
%K POMME ; APEX ; VITRIFICATION ; ENCAPSULATION
%N 2
%P 121-130
%U https://www.documentation.ird.fr/hor/fdi:010018692
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010018692.pdf
%V 20
%W Horizon (IRD)
%X In this paper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing in vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. Cold-hardening mother plants for 3 weeks at 5°C improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. Increasing the time during which mother-plants were maintained on standard medium without subculture before sampling of apices decreased the water content of shoot tips from about 85-88% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulation-dehydration technique. Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apcies, thus simplyfying the protocol. This simplified encapsulation-dehydration protocol was successfully applied to 11 out of the 12 cultivars tested. (Résumé d'auteur)
%$ 084VITRO ; 076AMEPLA02