@article{fdi:010018692,
  title = {{C}ryopreservation of apple shoot tips : importance of cryopreservation technique and of conditioning of donor plants},
  author = {{W}u, {Y}. and {E}ngelmann, {F}lorent and {Z}hao, {Y}. and {Z}hou, {M}. and {C}hen, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this paper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing in vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. {C}old-hardening mother plants for 3 weeks at 5°{C} improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. {I}ncreasing the time during which mother-plants were maintained on standard medium without subculture before sampling of apices decreased the water content of shoot tips from about 85-88% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. {T}he best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulation-dehydration technique. {U}sing apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apcies, thus simplyfying the protocol. {T}his simplified encapsulation-dehydration protocol was successfully applied to 11 out of the 12 cultivars tested. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CRYOCONSERVATION} ; {CONDITIONNEMENT} ; {PLANTE} ; {REGENERATION} ; {METHODOLOGIE} ; {ETUDE} {COMPARATIVE} ; {MATERIEL} {VEGETAL} ; {POMME} ; {APEX} ; {VITRIFICATION} ; {ENCAPSULATION}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {20},
  numero = {2},
  pages = {121--130},
  ISSN = {0143-2044},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018692},
}