Zhao Y., Wu Y., Engelmann Florent, Zhou M., Chen S. (1999). Cryopreservation of apple in vitro shoot tips by the droplet freezing method. Cryo-Letters, 20 (2), p. 109-112. ISSN 0143-2044.
Titre du document
Cryopreservation of apple in vitro shoot tips by the droplet freezing method
Année de publication
1999
Type de document
Article
Auteurs
Zhao Y., Wu Y., Engelmann Florent, Zhou M., Chen S.
Source
Cryo-Letters, 1999,
20 (2), p. 109-112 ISSN 0143-2044
Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. After 3 weeks of cold-hardening at 5°C, the shoot tips were precultured in liquid medium with 1.0% DMSO for 24 h at 5°C, treated for 75 min. at room temperature with 0.3 M sucrose and 15% DMSO, placed in 5 micrometer droplets of cryoprotectant medium containing 0.3 M sucrose and 15% DMSO on aluminium foil and cooled to -40°C at 0.2°C/min before immersion in liquid nitrogen. Recovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. Regrowth of frozen shoot tips was direct, without callus formation. (Résumé d'auteur)
Plan de classement
Collection, conservation [076AMEPLA02]
;
Culture végétale in vitro [084VITRO]
