%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Zhao, Y.
%A Wu, Y.
%A Engelmann, Florent
%A Zhou, M.
%A Chen, S.
%T Cryopreservation of apple in vitro shoot tips by the droplet freezing method
%D 1999
%L fdi:010018690
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K CULTURE IN VITRO ; CRYOCONSERVATION ; PLANTULE ; REGENERATION ; ETUDE EXPERIMENTALE ; METHODOLOGIE
%K POMME ; APEX
%N 2
%P 109-112
%U https://www.documentation.ird.fr/hor/fdi:010018690
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010018690.pdf
%V 20
%W Horizon (IRD)
%X Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. After 3 weeks of cold-hardening at 5°C, the shoot tips were precultured in liquid medium with 1.0% DMSO for 24 h at 5°C, treated for 75 min. at room temperature with 0.3 M sucrose and 15% DMSO, placed in 5 micrometer droplets of cryoprotectant medium containing 0.3 M sucrose and 15% DMSO on aluminium foil and cooled to -40°C at 0.2°C/min before immersion in liquid nitrogen. Recovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. Regrowth of frozen shoot tips was direct, without callus formation. (Résumé d'auteur)
%$ 084VITRO ; 076AMEPLA02