@article{fdi:010018690,
  title = {{C}ryopreservation of apple in vitro shoot tips by the droplet freezing method},
  author = {{Z}hao, {Y}. and {W}u, {Y}. and {E}ngelmann, {F}lorent and {Z}hou, {M}. and {C}hen, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}hoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. {A}fter 3 weeks of cold-hardening at 5°{C}, the shoot tips were precultured in liquid medium with 1.0% {DMSO} for 24 h at 5°{C}, treated for 75 min. at room temperature with 0.3 {M} sucrose and 15% {DMSO}, placed in 5 micrometer droplets of cryoprotectant medium containing 0.3 {M} sucrose and 15% {DMSO} on aluminium foil and cooled to -40°{C} at 0.2°{C}/min before immersion in liquid nitrogen. {R}ecovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. {R}egrowth of frozen shoot tips was direct, without callus formation. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CRYOCONSERVATION} ; {PLANTULE} ; {REGENERATION} ; {ETUDE} {EXPERIMENTALE} ; {METHODOLOGIE} ; {POMME} ; {APEX}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {20},
  numero = {2},
  pages = {109--112},
  ISSN = {0143-2044},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018690},
}