Khbaya B., Neyra Marc, Normand P., Zerhari K., Filali-Maltouf A. (1998). Genetic diversity and phylogeny of Rhizobia that nodulate Acacia spp. in Morocco assessed by analysis of rRNA genes. Applied and Environmental Microbiology, 64 (12), p. 4912-4917. ISSN 0099-2240.
Titre du document
Genetic diversity and phylogeny of Rhizobia that nodulate Acacia spp. in Morocco assessed by analysis of rRNA genes
Année de publication
1998
Type de document
Article
Auteurs
Khbaya B., Neyra Marc, Normand P., Zerhari K., Filali-Maltouf A.
Source
Applied and Environmental Microbiology, 1998,
64 (12), p. 4912-4917 ISSN 0099-2240
Forty rhizobia nodulating four Acacia species (A. gummifera, A. raddiana, A. cyanophylla, and A. horrida) were isolated from different sites in Morocco. These rhizobia were compared by analyzing both the 16S rRNA gene (rDNA) and the 16S-23S rRNA spacer by PCR with restriction fragment length polymorphism (RFLP) analysis. Analysis of the length of 16S-23S spacer showed a considerable diversity within these microsymbionts, but RFLP analysis of the amplified spacer revealed no additional heterogeneity. Three clusters were identified when 16S rDNA analysis was carried out. Two of these clusters include some isolates which nodulate, nonspecifically, the four Acacia species. These clusters, A and B, fit within the Sinorhizobium lineage and are closely related to S. meliloti and S. fredii, respectively. The third cluster appeared to belong to the Agrobacterium-Rhizobium galegae phylum and is more closely related to the Agrobacterium tumefaciens species. These relations were confirmed by sequencing a representative strain from each cluster. (Résumé d'auteur)
