Khbaya B. auteur aut IRD Neyra Marc auteur aut IRD Normand P. auteur aut IRD Zerhari K. auteur aut IRD Filali-Maltouf A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Forty rhizobia nodulating four Acacia species (A. gummifera, A. raddiana, A. cyanophylla, and A. horrida) were isolated from different sites in Morocco. These rhizobia were compared by analyzing both the 16S rRNA gene (rDNA) and the 16S-23S rRNA spacer by PCR with restriction fragment length polymorphism (RFLP) analysis. Analysis of the length of 16S-23S spacer showed a considerable diversity within these microsymbionts, but RFLP analysis of the amplified spacer revealed no additional heterogeneity. Three clusters were identified when 16S rDNA analysis was carried out. Two of these clusters include some isolates which nodulate, nonspecifically, the four Acacia species. These clusters, A and B, fit within the Sinorhizobium lineage and are closely related to S. meliloti and S. fredii$, respectively. The third cluster appeared to belong to the Agrobacterium-Rhizobium galegae phylum and is more closely related to the Agrobacterium tumefaciens$ species. These relations were confirmed by sequencing a representative strain from each cluster. (Résumé d'auteur) specialized SYMBIOSE NODULE LEGUMINEUSE BACTERIE DIVERSITE GENETIQUE PHYLOGENIE TECHNIQUE PCR ELECTROPHORESE MAROC 084SYMBIO01 64 12 4912-4917 1998 0099-2240 https://www.documentation.ird.fr/hor/fdi:010016790 0099-2240 [F B010016790] https://www.documentation.ird.fr/hor/fdi:010016790 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_53-54/010016790.pdf IRD - Base Horizon / Pleins textes 1999-02-01 2018-06-19 fdi:010016790 fre