@article{fdi:010016790,
  title = {{G}enetic diversity and phylogeny of {R}hizobia that nodulate {A}cacia spp. in {M}orocco assessed by analysis of r{RNA} genes},
  author = {{K}hbaya, {B}. and {N}eyra, {M}arc and {N}ormand, {P}. and {Z}erhari, {K}. and {F}ilali-{M}altouf, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}orty rhizobia nodulating four #{A}cacia$ species (#{A}. gummifera$, #{A}. raddiana$, #{A}. cyanophylla$, and #{A}. horrida$) were isolated from different sites in {M}orocco. {T}hese rhizobia were compared by analyzing both the 16{S} r{RNA} gene (r{DNA}) and the 16{S}-23{S} r{RNA} spacer by {PCR} with restriction fragment length polymorphism ({RFLP}) analysis. {A}nalysis of the length of 16{S}-23{S} spacer showed a considerable diversity within these microsymbionts, but {RFLP} analysis of the amplified spacer revealed no additional heterogeneity. {T}hree clusters were identified when 16{S} r{DNA} analysis was carried out. {T}wo of these clusters include some isolates which nodulate, nonspecifically, the four #{A}cacia$ species. {T}hese clusters, {A} and {B}, fit within the #{S}inorhizobium$ lineage and are closely related to #{S}. meliloti$ and {S}. fredii$, respectively. {T}he third cluster appeared to belong to the #{A}grobacterium$-#{R}hizobium galegae$ phylum and is more closely related to the {A}grobacterium tumefaciens$ species. {T}hese relations were confirmed by sequencing a representative strain from each cluster. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{SYMBIOSE} ; {NODULE} ; {LEGUMINEUSE} ; {BACTERIE} ; {DIVERSITE} {GENETIQUE} ; {PHYLOGENIE} ; {TECHNIQUE} {PCR} ; {ELECTROPHORESE} ; {MAROC}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {64},
  numero = {12},
  pages = {4912--4917},
  ISSN = {0099-2240},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010016790},
}