Brisse Sylvain, Barnabé Christian, Banuls Anne-Laure, Sidibé I., Noël S., Tibayrenc Michel. (1998). A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting. Molecular and Biochemical Parasitology, 92 (2), p. 253-263. ISSN 0166-6851.
Titre du document
A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting
Année de publication
1998
Type de document
Article
Auteurs
Brisse Sylvain, Barnabé Christian, Banuls Anne-Laure, Sidibé I., Noël S., Tibayrenc Michel
Source
Molecular and Biochemical Parasitology, 1998,
92 (2), p. 253-263 ISSN 0166-6851
We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data gave congruent phylogenetic results. The CL Brener reference strain fell into the second major phylogenetic subdivision of T. cruzi, and was genetically very close to the Tulahuen reference strain. No reliable RAPD character and only one MLEE character permitted us to distinguish between the CL Brener and Tulahuen reference strain. In contrast, many RAPD and MLEE characters were able to distinguish between the CL Brener reference strain and the other T. cruzi genotypes analyzed here, in particular the formerly described principal zymodemes I, II and III. It is suspected that both CL Brener and Tulahuen are hybrid genotypes, a fact that should be taken into account when interpreting sequence data. Moreover, our study confirms that the species T. cruzi is genetically very heterogeneous. We recommend future comparison of sequencing data from the CL Brener reference strain with those of at least one radically distinct T. cruzi genotype, belonging to the other major phylogenetic subdivision of this species. (Résumé d'auteur)
Plan de classement
Glossines, réduviides et trypanosomiases [052GLOTRY]
