%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Brisse, Sylvain
%A Barnabé, Christian
%A Banuls, Anne-Laure
%A Sidibé, I.
%A Noël, S.
%A Tibayrenc, Michel
%T A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting
%D 1998
%L fdi:010013980
%G ENG
%J Molecular and Biochemical Parasitology
%@ 0166-6851
%K MALADIE DE CHAGAS ; AGENT PATHOGENE ; CLONE ; GENOTYPE ; PHYLOGENIE ; ELECTROPHORESE ; TECHNIQUE RAPD
%K CARACTERISATION GENETIQUE
%N 2
%P 253-263
%R 10.1016/S0166-6851(98)00005-X
%U https://www.documentation.ird.fr/hor/fdi:010013980
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010013980.pdf
%V 92
%W Horizon (IRD)
%X We have assessed the phylogenetic status of the #Trypanosoma cruzi$ Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of #T. cruzi$. MLEE and RAPD data gave congruent phylogenetic results. The CL Brener reference strain fell into the second major phylogenetic subdivision of #T. cruzi$, and was genetically very close to the Tulahuen reference strain. No reliable RAPD character and only one MLEE character permitted us to distinguish between the CL Brener and Tulahuen reference strain. In contrast, many RAPD and MLEE characters were able to distinguish between the CL Brener reference strain and the other #T. cruzi$ genotypes analyzed here, in particular the formerly described principal zymodemes I, II and III. It is suspected that both CL Brener and Tulahuen are hybrid genotypes, a fact that should be taken into account when interpreting sequence data. Moreover, our study confirms that the species #T. cruzi$ is genetically very heterogeneous. We recommend future comparison of sequencing data from the CL Brener reference strain with those of at least one radically distinct #T. cruzi$ genotype, belonging to the other major phylogenetic subdivision of this species. (Résumé d'auteur)
%$ 052GLOTRY