Gonzalez-Arnao M.T., Engelmann Florent, Urra C., Morenza M., Rios A. (1998). Cryopreservation of citrus apices using the encapsulation dehydration technique. Cryo-Letters, 19 (3), p. 177-182. ISSN 0143-2044.
Titre du document
Cryopreservation of citrus apices using the encapsulation dehydration technique
Année de publication
1998
Type de document
Article
Auteurs
Gonzalez-Arnao M.T., Engelmann Florent, Urra C., Morenza M., Rios A.
Source
Cryo-Letters, 1998,
19 (3), p. 177-182 ISSN 0143-2044
Cryopreservation of apices sampled on in vitro plantlets of Poncirus trifoliata (L.) Raf. was achieved using the encapsulation-dehydration technique. The highest survival rates after cryopreservation (up to 50%) were obtained when encapsulated apices were pregrown for 3 d either directly in liquid medium containing 0.5 M sucrose or in media with progressively increasing sucrose concentration from 0.3 to 0.75 M, desiccated to 20-25% moisture content and frozen slowly (0.5°C/min) from +20°C to -40°C before immersion in liquid nitrogen. Recovery of whole plantlets from cryopreserved apices took place directly, without transitory callus formation. (Résumé d'auteur)
Plan de classement
Culture in vitro [076AMEPLA07]
;
Culture végétale in vitro [084VITRO]
Descripteurs
CULTURE IN VITRO ; CRYOCONSERVATION ; DESHYDRATATION ; APEX
