@article{fdi:010013976,
  title = {{C}ryopreservation of citrus apices using the encapsulation dehydration technique},
  author = {{G}onzalez-{A}rnao, {M}.{T}. and {E}ngelmann, {F}lorent and {U}rra, {C}. and {M}orenza, {M}. and {R}ios, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ryopreservation of apices sampled on in vitro plantlets of #{P}oncirus trifoliata$ ({L}.) {R}af. was achieved using the encapsulation-dehydration technique. {T}he highest survival rates after cryopreservation (up to 50%) were obtained when encapsulated apices were pregrown for 3 d either directly in liquid medium containing 0.5 {M} sucrose or in media with progressively increasing sucrose concentration from 0.3 to 0.75 {M}, desiccated to 20-25% moisture content and frozen slowly (0.5°{C}/min) from +20°{C} to -40°{C} before immersion in liquid nitrogen. {R}ecovery of whole plantlets from cryopreserved apices took place directly, without transitory callus formation. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CRYOCONSERVATION} ; {DESHYDRATATION} ; {APEX}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {19},
  numero = {3},
  pages = {177--182},
  ISSN = {0143-2044},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010013976},
}