%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Gonzalez-Arnao, M.T.
%A Engelmann, Florent
%A Urra, C.
%A Morenza, M.
%A Rios, A.
%T Cryopreservation of citrus apices using the encapsulation dehydration technique
%D 1998
%L fdi:010013976
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K CULTURE IN VITRO ; CRYOCONSERVATION ; DESHYDRATATION
%K APEX
%N 3
%P 177-182
%U https://www.documentation.ird.fr/hor/fdi:010013976
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010013976.pdf
%V 19
%W Horizon (IRD)
%X Cryopreservation of apices sampled on in vitro plantlets of #Poncirus trifoliata$ (L.) Raf. was achieved using the encapsulation-dehydration technique. The highest survival rates after cryopreservation (up to 50%) were obtained when encapsulated apices were pregrown for 3 d either directly in liquid medium containing 0.5 M sucrose or in media with progressively increasing sucrose concentration from 0.3 to 0.75 M, desiccated to 20-25% moisture content and frozen slowly (0.5°C/min) from +20°C to -40°C before immersion in liquid nitrogen. Recovery of whole plantlets from cryopreserved apices took place directly, without transitory callus formation. (Résumé d'auteur)
%$ 084VITRO ; 076AMEPLA07