Malaurie Bernard, Trouslot Marie-France, Engelmann Florent, Chabrillange Nathalie. (1998). Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata 'Brazo Fuerte' and D. bulbifera 'Nouméa Imboro') shoot apices by encapsulation-dehydration. Cryo-Letters, 19 (1), p. 15-26. ISSN 0143-2044.
Titre du document
Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata 'Brazo Fuerte' and D. bulbifera 'Nouméa Imboro') shoot apices by encapsulation-dehydration
Cryo-Letters, 1998,
19 (1), p. 15-26 ISSN 0143-2044
Apical shoot tips of in vitro plantlets of two edible yam species were subjected to a wide range of pretreatment conditions prior to cryopreservation using the encapsulation-dehydration technique. For D. alata, the highest survival rate (up to 67%) was obtained after a 3 to 10-day pretreatment with 0.9M and 1M sucrose followed by 11 to 16 h. of silica gel-dehydration, rapid cooling in liquid nitrogen and, after rewarming, subculture for 35 d. on medium supplemented with growth regulators. For D. bulbifera, optimal survival (up to 65%) was achieved following pretreatment with sucrose concentrations higher than 0.75M, regardless of the pretreatment duration, and 14 to 16 h of dehydration. Recovery of frozen apices into plantlets was achieved after three months of culture on growth regulator-free medium, at a rate of 19% and 60% for D. alata and D. bulbifera, respectively. (Résumé d'auteur)
Plan de classement
Culture in vitro [076AMEPLA07]
;
Culture végétale in vitro [084VITRO]
Descripteurs
AMELIORATION DES PLANTES ; CULTURE IN VITRO ; DESHYDRATATION ; CONGELATION ; CRYOCONSERVATION
