@article{fdi:010012669,
  title = {{E}ffect of pretreatment conditions on the cryopreservation of in vitro-cultured yam ({D}ioscorea alata '{B}razo {F}uerte' and {D}. bulbifera '{N}oum{\'e}a {I}mboro') shoot apices by encapsulation-dehydration},
  author = {{M}alaurie, {B}ernard and {T}rouslot, {M}arie-{F}rance and {E}ngelmann, {F}lorent and {C}habrillange, {N}athalie},
  editor = {},
  language = {{ENG}},
  abstract = {{A}pical shoot tips of in vitro plantlets of two edible yam species were subjected to a wide range of pretreatment conditions prior to cryopreservation using the encapsulation-dehydration technique. {F}or #{D}. alata$, the highest survival rate (up to 67%) was obtained after a 3 to 10-day pretreatment with 0.9{M} and 1{M} sucrose followed by 11 to 16 h. of silica gel-dehydration, rapid cooling in liquid nitrogen and, after rewarming, subculture for 35 d. on medium supplemented with growth regulators. {F}or #{D}. bulbifera$, optimal survival (up to 65%) was achieved following pretreatment with sucrose concentrations higher than 0.75{M}, regardless of the pretreatment duration, and 14 to 16 h of dehydration. {R}ecovery of frozen apices into plantlets was achieved after three months of culture on growth regulator-free medium, at a rate of 19% and 60% for #{D}. alata$ and #{D}. bulbifera$, respectively. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{AMELIORATION} {DES} {PLANTES} ; {CULTURE} {IN} {VITRO} ; {DESHYDRATATION} ; {CONGELATION} ; {CRYOCONSERVATION}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {19},
  numero = {1},
  pages = {15--26},
  ISSN = {0143-2044},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010012669},
}