%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Malaurie, Bernard
%A Trouslot, Marie-France
%A Engelmann, Florent
%A Chabrillange, Nathalie
%T Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata 'Brazo Fuerte' and D. bulbifera 'Nouméa Imboro') shoot apices by encapsulation-dehydration
%D 1998
%L fdi:010012669
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K AMELIORATION DES PLANTES ; CULTURE IN VITRO ; DESHYDRATATION ; CONGELATION ; CRYOCONSERVATION
%N 1
%P 15-26
%U https://www.documentation.ird.fr/hor/fdi:010012669
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010012669.pdf
%V 19
%W Horizon (IRD)
%X Apical shoot tips of in vitro plantlets of two edible yam species were subjected to a wide range of pretreatment conditions prior to cryopreservation using the encapsulation-dehydration technique. For #D. alata$, the highest survival rate (up to 67%) was obtained after a 3 to 10-day pretreatment with 0.9M and 1M sucrose followed by 11 to 16 h. of silica gel-dehydration, rapid cooling in liquid nitrogen and, after rewarming, subculture for 35 d. on medium supplemented with growth regulators. For #D. bulbifera$, optimal survival (up to 65%) was achieved following pretreatment with sucrose concentrations higher than 0.75M, regardless of the pretreatment duration, and 14 to 16 h of dehydration. Recovery of frozen apices into plantlets was achieved after three months of culture on growth regulator-free medium, at a rate of 19% and 60% for #D. alata$ and #D. bulbifera$, respectively. (Résumé d'auteur)
%$ 076AMEPLA07 ; 084VITRO