Djigo O. K. M., Gomez N., Salem Msoa, Basco Leonardo, Boukhary Aoms, Briolant S. (2023). Performance of a commercial multiplex allele-specific polymerase chain reaction kit to genotype African-type glucose-6-phosphate dehydrogenase deficiency. American Journal of Tropical Medicine and Hygiene, 108 (2), p. 449-455. ISSN 0002-9637.
Titre du document
Performance of a commercial multiplex allele-specific polymerase chain reaction kit to genotype African-type glucose-6-phosphate dehydrogenase deficiency
Djigo O. K. M., Gomez N., Salem Msoa, Basco Leonardo, Boukhary Aoms, Briolant S.
Source
American Journal of Tropical Medicine and Hygiene, 2023,
108 (2), p. 449-455 ISSN 0002-9637
8-Aminoquinoline antimalarial drugs (primaquine, tafenoquine) are required for complete cure of Plasmodium vivax malaria, but they are contraindicated in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. In the absence of spectrophotometry, which is a gold standard for measuring G6PD activity, G6PD genotyping is one of the alternatives to establish a database and distribution map of G6PD enzyme deficiency in Mauritania, which has become a new epicenter of P. vivax malaria in West Africa. The aim of our study was to assess the performance of multiplex allele-specific polymerase chain reaction (PCR) (African-type Diaplex CTM G6PD kit) against PCR-restriction fragment length polymorphism and sequencing. Of 146 mutations associated with G6PD A- genotypes in 177 blood samples from Mauritanian patients, all but two samples were identified correctly using multiplex allele-specific PCR (100% sensitivity and 99% specificity; "almost perfect agreement" between allele-specific PCR and PCR-restriction frag-ment length polymorphism/sequencing, with a kappa coefficient of 0.977). Despite a suboptimal PCR protocol for dried blood spots and the inability of the commercial assay to predict unequivocally the G6PD enzyme level in heterozygous females, the African-type Diaplex CTM G6PD genotyping kit seemed to be a valuable screening tool for male subjects and for research purposes in resource-limited countries where spectrophotometer and DNA sequencing are not available.
