%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Djigo, O. K. M.
%A Gomez, N.
%A Salem, Msoa
%A Basco, Leonardo
%A Boukhary, Aoms
%A Briolant, S.
%T Performance of a commercial multiplex allele-specific polymerase chain reaction kit to genotype African-type glucose-6-phosphate dehydrogenase deficiency
%D 2023
%L PAR00025898
%G ENG
%J American Journal of Tropical Medicine and Hygiene
%@ 0002-9637
%M ISI:000952282900035
%N 2
%P 449-455
%R 10.4269/ajtmh.21-1081
%U https://www.documentation.ird.fr/hor/PAR00025898
%> https://www.documentation.ird.fr/intranet/publi/2023-05/010087552.pdf
%V 108
%W Horizon (IRD)
%X 8-Aminoquinoline antimalarial drugs (primaquine, tafenoquine) are required for complete cure of Plasmodium vivax malaria, but they are contraindicated in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. In the absence of spectrophotometry, which is a gold standard for measuring G6PD activity, G6PD genotyping is one of the alternatives to establish a database and distribution map of G6PD enzyme deficiency in Mauritania, which has become a new epicenter of P. vivax malaria in West Africa. The aim of our study was to assess the performance of multiplex allele-specific polymerase chain reaction (PCR) (African-type Diaplex CTM G6PD kit) against PCR-restriction fragment length polymorphism and sequencing. Of 146 mutations associated with G6PD A- genotypes in 177 blood samples from Mauritanian patients, all but two samples were identified correctly using multiplex allele-specific PCR (100% sensitivity and 99% specificity; "almost perfect agreement" between allele-specific PCR and PCR-restriction frag-ment length polymorphism/sequencing, with a kappa coefficient of 0.977). Despite a suboptimal PCR protocol for dried blood spots and the inability of the commercial assay to predict unequivocally the G6PD enzyme level in heterozygous females, the African-type Diaplex CTM G6PD genotyping kit seemed to be a valuable screening tool for male subjects and for research purposes in resource-limited countries where spectrophotometer and DNA sequencing are not available.
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