@article{PAR00025898,
  title = {{P}erformance of a commercial multiplex allele-specific polymerase chain reaction kit to genotype {A}frican-type glucose-6-phosphate dehydrogenase deficiency},
  author = {{D}jigo, {O}. {K}. {M}. and {G}omez, {N}. and {S}alem, {M}soa and {B}asco, {L}eonardo and {B}oukhary, {A}oms and {B}riolant, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {8-{A}minoquinoline antimalarial drugs (primaquine, tafenoquine) are required for complete cure of {P}lasmodium vivax malaria, but they are contraindicated in patients with glucose-6-phosphate dehydrogenase ({G}6{PD}) deficiency. {I}n the absence of spectrophotometry, which is a gold standard for measuring {G}6{PD} activity, {G}6{PD} genotyping is one of the alternatives to establish a database and distribution map of {G}6{PD} enzyme deficiency in {M}auritania, which has become a new epicenter of {P}. vivax malaria in {W}est {A}frica. {T}he aim of our study was to assess the performance of multiplex allele-specific polymerase chain reaction ({PCR}) ({A}frican-type {D}iaplex {CTM} {G}6{PD} kit) against {PCR}-restriction fragment length polymorphism and sequencing. {O}f 146 mutations associated with {G}6{PD} {A}- genotypes in 177 blood samples from {M}auritanian patients, all but two samples were identified correctly using multiplex allele-specific {PCR} (100% sensitivity and 99% specificity; "almost perfect agreement" between allele-specific {PCR} and {PCR}-restriction frag-ment length polymorphism/sequencing, with a kappa coefficient of 0.977). {D}espite a suboptimal {PCR} protocol for dried blood spots and the inability of the commercial assay to predict unequivocally the {G}6{PD} enzyme level in heterozygous females, the {A}frican-type {D}iaplex {CTM} {G}6{PD} genotyping kit seemed to be a valuable screening tool for male subjects and for research purposes in resource-limited countries where spectrophotometer and {DNA} sequencing are not available.},
  keywords = {},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {108},
  numero = {2},
  pages = {449--455},
  ISSN = {0002-9637},
  year = {2023},
  DOI = {10.4269/ajtmh.21-1081},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00025898},
}