Nagot N., Foulongne V., Becquart P., Mayaud P., Konate I., Ouedraogo A., Defer M. C., Weiss H., Van de Perre P., Segondy M. (2005). Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women. Jaids Journal of Acquired Immune Deficiency Syndromes, 39 (5), p. 632-634. ISSN 1525-4135.
Titre du document
Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women
Nagot N., Foulongne V., Becquart P., Mayaud P., Konate I., Ouedraogo A., Defer M. C., Weiss H., Van de Perre P., Segondy M.
Source
Jaids Journal of Acquired Immune Deficiency Syndromes, 2005,
39 (5), p. 632-634 ISSN 1525-4135
Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.
