%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Nagot, N.
%A Foulongne, V.
%A Becquart, P.
%A Mayaud, P.
%A Konate, I.
%A Ouedraogo, A.
%A Defer, M. C.
%A Weiss, H.
%A Van de Perre, P.
%A Segondy, M.
%T Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women
%D 2005
%L PAR00000396
%G ENG
%J Jaids Journal of Acquired Immune Deficiency Syndromes
%@ 1525-4135
%K HIV 1 RNA ; HSV 2 DNA ; genital shedding ; African women
%M CC:0002309765-0020
%N 5
%P 632-634
%U https://www.documentation.ird.fr/hor/PAR00000396
%V 39
%W Horizon (IRD)
%X Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.