Miché L., Dries A., Ben Ammar I., Davidson Sylvain, Cagnacci Loris, Combet-Blanc Yannick, Abecassis V., Penton Fernandez G., Christen Pierre. (2024). Changes in chemical properties and microbial communities' composition of a forest litter-based biofertilizer produced through aerated solid-state culture under different oxygen conditions. Environmental Science and Pollution Research, 32 (28), 16725-16739. ISSN 1614-7499.
Titre du document
Changes in chemical properties and microbial communities' composition of a forest litter-based biofertilizer produced through aerated solid-state culture under different oxygen conditions
Année de publication
2024
Auteurs
Miché L., Dries A., Ben Ammar I., Davidson Sylvain, Cagnacci Loris, Combet-Blanc Yannick, Abecassis V., Penton Fernandez G., Christen Pierre
Source
Environmental Science and Pollution Research, 2024,
32 (28), 16725-16739 ISSN 1614-7499
Fermented forest litter (FFL) is a bioproduct used as biofertilizer for several decades in Eastern Asia and Latin America. It
is locally handcrafted by farmers in anaerobic conditions by fermenting forest litter added with agricultural by-products such
as whey, cereal bran, and molasses. The aim of this study was to characterize the FFL process and product through gas and
liquid chromatography analyses. It also provides some highlights on the influence of O 2 on this solid-state culture. Under
anoxic condition, a maximum CO 2 production rate (CDPR) of 0.41 mL/h?g dry matter (dm) was reached after 8 days. The
main volatile organic compounds (VOCs) were ethanol and ethyl acetate, with a production rate profile similar to CDPR.
After 21 days of culture, no residual sucrose nor lactose was detected. Lactic and acetic acids reached 58.8 mg/g dm and
10.2 mg/g dm, respectively, ensuring the acidification of the matrix to a final pH of 4.72. A metabarcoding analysis revealed
that heterolactic acid bacteria (Lentilactobacillus, Leuconostoc), homolactic acid bacteria (Lactococcus), and yeasts (Sac-
charomyces, Clavispora) were predominant. Predicted genes in the microbiome confirmed the potential link between detected
bacteria and acids and VOCs produced. When O 2 was fed to the cultures, final pH reached values up to 8.5. No significant
amounts of lactic nor acetic acid were found. In addition, a strong shift in microbial communities was observed, with a
predominance of Proteobacteria and molds, among which are potential pathogens like Fusarium species. This suggests that
particular care must be brought to maintain anoxic conditions throughout the process.
Plan de classement
Biotechnologies [084]
;
Economie et sociologie rurale [098]
Localisation
Fonds IRD [F B010095706]
Identifiant IRD
fdi:010095706
