Yadouleton A., Nouatin O., Kissira I., Houngbegnon P., Cottrell Gilles, Fievet Nadine, Sohou S., Butel Christelle, Serrano Laetitia, Guichet E., Vidal Nicole, Delaporte E., Ayouba Ahidjo, Peeters Martine, Massougbodji A. (2024). Genomic surveillance of virus in Benin. Infection Genetics and Evolution, 125, p. 105674 [6 p.]. ISSN 1567-1348.
Titre du document
Genomic surveillance of virus in Benin
Année de publication
2024
Auteurs
Yadouleton A., Nouatin O., Kissira I., Houngbegnon P., Cottrell Gilles, Fievet Nadine, Sohou S., Butel Christelle, Serrano Laetitia, Guichet E., Vidal Nicole, Delaporte E., Ayouba Ahidjo, Peeters Martine, Massougbodji A.
Source
Infection Genetics and Evolution, 2024,
125, p. 105674 [6 p.] ISSN 1567-1348
Objective: Dengue is a widespread viral infection transmitted from mosquitoes to humans, mainly in tropical and subtropical climates. In Benin, only dengue virus (DENV) serotype 2 infection has been previously described in humans. This study aimed to investigate DENV infection and serotypes in suspected patients. Methods: Plasma samples from 464 patients attending health centers in February 2023 with clinical symptoms and suspected for dengue infection were included, and analyzed for DENV by real time quantitative Polymerase Chain Reaction (Dengue Altona 3.0 kit). PCR positives samples were further characterized by whole genome sequencing and phylogenetic analysis to identify the circulating DENV serotype. Results: The RT-qPCR results showed that four patients (D6, D23, D28, D44) were positive with the cycle threshold values less than 40 (31.3, 34.7, 14.7 and 14.3) respectively. Full-length DENV sequences were obtained for D6, D28 and D44. One patient (D6) was infected with DENV-1 serotype, and the two others (D28 and D44) were positive for DENV-3. Phylogenetic analysis shows that the new DENV-1 sequence is close to those obtained in Burkina Faso in 2022 and Nigeria in 2023, and the two DENV-3 sequences form a separate cluster with sequences obtained in Burkina Faso in 2022. Conclusion: We showed for the first time, the presence of dengue serotype 1 and serotype 3 infection in Benin. These results send a strong signal to health authorities and show that arbovirus surveillance efforts must be integrated into pathogen monitoring programs.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
BENIN
Localisation
Fonds IRD [F B010091947]
Identifiant IRD
fdi:010091947
