Commans Florian, Hayer Juliette, Do B. N., Tran T. T. T., Le T. T. H., Bui T. T., Le H. S., Banuls Anne-Laure, Bui T. S., Nguyen Q. H. (2024). Whole-genome sequence and resistance determinants of four Elizabethkingia anophelis clinical isolates collected in Hanoi, Vietnam. Scientific Reports - Nature, 14 (1), p. 7241 [16 p.]. ISSN 2045-2322.
Titre du document
Whole-genome sequence and resistance determinants of four Elizabethkingia anophelis clinical isolates collected in Hanoi, Vietnam
Année de publication
2024
Auteurs
Commans Florian, Hayer Juliette, Do B. N., Tran T. T. T., Le T. T. H., Bui T. T., Le H. S., Banuls Anne-Laure, Bui T. S., Nguyen Q. H.
Source
Scientific Reports - Nature, 2024,
14 (1), p. 7241 [16 p.] ISSN 2045-2322
Four isolates of the opportunistic pathogen Elizabethkingia anophelis were identified for the first time in a Vietnamese hospital and underwent antimicrobial susceptibility testing and genomic characterization by whole-genome sequencing. Complete, fully circularized genome sequences were obtained for all four isolates. Average Nucleotide Identity analysis and single nucleotide polymorphism phylogenetic analysis on the core genome showed that three of the four isolates were genetically distinct, ruling out the hypothesis of a single strain emergence. Antibiotic susceptibility testing highlighted multi-resistant phenotypes against most antimicrobial families, including beta-lactams, carbapenems, aminoglycosides, quinolones, macrolides, amphenicols, rifamycins and glycopeptides. Additionally, in silico genomic analysis was used to correlate the phenotypic susceptibility to putative resistance determinants, including resistance genes, point mutations and multidrug efflux pumps. Nine different resistance genes were located inside a single resistance pocket predicted to be a putative Integrative and Conjugative Element (ICE). This novel ICE was shared by three isolates from two different lineages and displayed similarity with ICEs previously reported in various Elizabethkingia and Chryseobacterium species. The role of such ICEs in pathogenicity, genome plasticity and antimicrobial resistance gene spread within the Flavobacteriaceae family needs to be further elucidated.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
;
Biotechnologies [084]
Description Géographique
VIET NAM
Localisation
Fonds IRD [F B010090560]
Identifiant IRD
fdi:010090560
