Sanders J. G., Yan W. W., Mjungu D., Lonsdorf E. V., Hart J. A., Sanz C. M., Morgan D. B., Peeters Martine, Hahn B. H., Moeller A. H. (2022). A low-cost genomics workflow enables isolate screening and strain-level analyses within microbiomes. Genome Biology, 23 (1), p. 212 [17 p.]. ISSN 1474-760X.
Titre du document
A low-cost genomics workflow enables isolate screening and strain-level analyses within microbiomes
Earth's environments harbor complex consortia of microbes that affect processes ranging from host health to biogeochemical cycles. Understanding their evolution and function is limited by an inability to isolate genomes in a high-throughput manner. Here, we present a workflow for bacterial whole-genome sequencing using open-source labware and the OpenTrons robotics platform, reducing costs to approximately 10 per genome. We assess genomic diversity within 45 gut bacterial species from wild-living chimpanzees and bonobos. We quantify intraspecific genomic diversity and reveal divergence of homologous plasmids between hosts. This enables population genetic analyses of bacterial strains not currently possible with metagenomic data alone.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde animal [080]
;
Biotechnologies [084]
Description Géographique
CAMEROUN ; CONGO ; TANZANIE ; REPUBLIQUE DEMOCRATIQUE DU CONGO
