@article{fdi:010086335, title = {{A} low-cost genomics workflow enables isolate screening and strain-level analyses within microbiomes}, author = {{S}anders, {J}. {G}. and {Y}an, {W}. {W}. and {M}jungu, {D}. and {L}onsdorf, {E}. {V}. and {H}art, {J}. {A}. and {S}anz, {C}. {M}. and {M}organ, {D}. {B}. and {P}eeters, {M}artine and {H}ahn, {B}. {H}. and {M}oeller, {A}. {H}.}, editor = {}, language = {{ENG}}, abstract = {{E}arth's environments harbor complex consortia of microbes that affect processes ranging from host health to biogeochemical cycles. {U}nderstanding their evolution and function is limited by an inability to isolate genomes in a high-throughput manner. {H}ere, we present a workflow for bacterial whole-genome sequencing using open-source labware and the {O}pen{T}rons robotics platform, reducing costs to approximately $10 per genome. {W}e assess genomic diversity within 45 gut bacterial species from wild-living chimpanzees and bonobos. {W}e quantify intraspecific genomic diversity and reveal divergence of homologous plasmids between hosts. {T}his enables population genetic analyses of bacterial strains not currently possible with metagenomic data alone.}, keywords = {{CAMEROUN} ; {CONGO} ; {TANZANIE} ; {REPUBLIQUE} {DEMOCRATIQUE} {DU} {CONGO}}, booktitle = {}, journal = {{G}enome {B}iology}, volume = {23}, numero = {1}, pages = {212 [17 p.]}, ISSN = {1474-760{X}}, year = {2022}, DOI = {10.1186/s13059-022-02777-w}, URL = {https://www.documentation.ird.fr/hor/fdi:010086335}, }