Wen M., Pan Q. W., Jouanno E., Montfort J., Zahm M., Cabau C., Klopp C., Iampietro C., Roques C., Bouchez O., Castinel A., Donnadieu C., Parrinello H., Poncet C., Belmonte E., Gautier V., Avarre Jean-Christophe, Dugué Rémi, Gustiano R., Ha T. T. T., Campet M., Sriphairoj K., Ribolli J., de Almeida F. L., Desvignes T., Postlethwait J. H., Bucao C. F., Robinson-Rechavi M., Bobe J., Herpin A., Guiguen Y. (2022). An ancient truncated duplication of the anti-Mullerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family. Molecular Ecology Resources, [Early access], p. [18 p.]. ISSN 1755-098X.
Titre du document
An ancient truncated duplication of the anti-Mullerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family
Année de publication
2022
Auteurs
Wen M., Pan Q. W., Jouanno E., Montfort J., Zahm M., Cabau C., Klopp C., Iampietro C., Roques C., Bouchez O., Castinel A., Donnadieu C., Parrinello H., Poncet C., Belmonte E., Gautier V., Avarre Jean-Christophe, Dugué Rémi, Gustiano R., Ha T. T. T., Campet M., Sriphairoj K., Ribolli J., de Almeida F. L., Desvignes T., Postlethwait J. H., Bucao C. F., Robinson-Rechavi M., Bobe J., Herpin A., Guiguen Y.
Source
Molecular Ecology Resources, 2022,
[Early access], p. [18 p.] ISSN 1755-098X
The evolution of sex determination (SD) in teleosts is amazingly dynamic, as reflected by the variety of different master sex-determining genes identified. Pangasiids are economically important catfishes in South Asian countries, but little is known about their SD system. Here, we generated novel genomic resources for 12 Pangasiids and characterized their SD system. Based on a Pangasianocion hypophthalmus chromosome-scale genome assembly, we identified an anti-Mullerian hormone receptor type II gene (amhr2) duplication, which was further characterized as being sex-linked in males and expressed only in testes. These results point to a Y chromosome male-specific duplication (amhr2by) of the autosomal amhr2a. Sequence annotation revealed that the P. hypophthalmus Amhr2by is truncated in its N-terminal domain, lacking the cysteine-rich extracellular part of the receptor that is crucial for ligand binding, suggesting a potential route for its neofunctionalization. Reference-guided assembly of 11 additional Pangasiids, along with sex-linkage studies, revealed that this truncated amhr2 by duplication is a male-specific conserved gene in Pangasiids. Reconstructions of the amhr2 phylogeny suggested that amhr2by arose from an ancient duplication/insertion event at the root of the Siluroidei radiation that is dated to similar to 100 million years ago. Together these results bring multiple lines of evidence supporting that amhr2by is an ancient and conserved master sex-determining gene in Pangasiids, a finding that highlights the recurrent use of the transforming growth factor beta pathway, which is often used for the recruitment of teleost master SD genes, and provides another empirical case towards firther understanding of dynamics of SD systems.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Limnologie biologique / Océanographie biologique [034]
Localisation
Fonds IRD [F B010084711]
Identifiant IRD
fdi:010084711
