de Haas P., Yenew B., Mengesha E., Slyzkyi A., Gashu Z., Lounnas Manon, Tesfaye E., Bedru A., Tiemersma E., Kremer K., Amare M., Diriba G., Zerihun B., Gudina T., Tegegn B., Bonnet Maryline, Negeri C., Klinkenberg E. (2021). The Simple One-Step (SOS) stool processing method for use with the Xpert MTB/RIF assay for a child-friendly diagnosis of tuberculosis closer to the point of care. Journal of Clinical Microbiology, 59 (8), e00406-21 [11 p.]. ISSN 0095-1137.
Titre du document
The Simple One-Step (SOS) stool processing method for use with the Xpert MTB/RIF assay for a child-friendly diagnosis of tuberculosis closer to the point of care
Année de publication
2021
Auteurs
de Haas P., Yenew B., Mengesha E., Slyzkyi A., Gashu Z., Lounnas Manon, Tesfaye E., Bedru A., Tiemersma E., Kremer K., Amare M., Diriba G., Zerihun B., Gudina T., Tegegn B., Bonnet Maryline, Negeri C., Klinkenberg E.
Source
Journal of Clinical Microbiology, 2021,
59 (8), e00406-21 [11 p.] ISSN 0095-1137
Young children cannot easily produce sputum for diagnosis of pulmonary tuberculosis (TB). Alternatively, Mycobacterium tuberculosis complex bacilli can be detected in stool by using the Xpert MTB/RIF (Ultra) assay (Xpert). Published stool processing methods contain somewhat complex procedures and require additional supplies. The aim of this study was to develop a simple one-step (SOS) stool processing method based on gravity sedimentation only, similar to Xpert testing of sputum samples, for the detection of M. tuberculosis in stool samples. We first assessed whether the SOS stool method could provide valid Xpert results without the need for bead-beating, dilution, and filtration steps. We concluded that this was the case, and we then validated the SOS stool method by testing spiked stool samples. By using the SOS stool method, 27 of the 29 spiked samples gave valid Xpert results, and M. tuberculosis was recovered from all 27 samples. The proof of principle of the SOS stool method was demonstrated in routine settings in Addis Ababa, Ethiopia. Nine of 123 children with presumptive TB had M. tuberculosis-positive results for nasogastric aspiration (NGA) samples, and 7 (77.8%) of those children also had M. tuberculosis-positive Xpert results for stool samples. Additionally, M. tuberculosis was detected in the stool samples but not the NGA samples from 2 children. The SOS stool processing method makes use of the standard Xpert assay kit, without the need for additional supplies or equipment. The method can potentially be rolled out to any Xpert site, bringing a bacteriologically confirmed diagnosis of TB in children closer to the point of care.
Plan de classement
Santé : généralités [050]
;
Biotechnologies [084]
Description Géographique
ETHIOPIE
Localisation
Fonds IRD [F B010083293]
Identifiant IRD
fdi:010083293
