@article{fdi:010083293,
  title = {{T}he {S}imple {O}ne-{S}tep ({SOS}) stool processing method for use with the {X}pert {MTB}/{RIF} assay for a child-friendly diagnosis of tuberculosis closer to the point of care},
  author = {de {H}aas, {P}. and {Y}enew, {B}. and {M}engesha, {E}. and {S}lyzkyi, {A}. and {G}ashu, {Z}. and {L}ounnas, {M}anon and {T}esfaye, {E}. and {B}edru, {A}. and {T}iemersma, {E}. and {K}remer, {K}. and {A}mare, {M}. and {D}iriba, {G}. and {Z}erihun, {B}. and {G}udina, {T}. and {T}egegn, {B}. and {B}onnet, {M}aryline and {N}egeri, {C}. and {K}linkenberg, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Y}oung children cannot easily produce sputum for diagnosis of pulmonary tuberculosis ({TB}). {A}lternatively, {M}ycobacterium tuberculosis complex bacilli can be detected in stool by using the {X}pert {MTB}/{RIF} ({U}ltra) assay ({X}pert). {P}ublished stool processing methods contain somewhat complex procedures and require additional supplies. {T}he aim of this study was to develop a simple one-step ({SOS}) stool processing method based on gravity sedimentation only, similar to {X}pert testing of sputum samples, for the detection of {M}. tuberculosis in stool samples. {W}e first assessed whether the {SOS} stool method could provide valid {X}pert results without the need for bead-beating, dilution, and filtration steps. {W}e concluded that this was the case, and we then validated the {SOS} stool method by testing spiked stool samples. {B}y using the {SOS} stool method, 27 of the 29 spiked samples gave valid {X}pert results, and {M}. tuberculosis was recovered from all 27 samples. {T}he proof of principle of the {SOS} stool method was demonstrated in routine settings in {A}ddis {A}baba, {E}thiopia. {N}ine of 123 children with presumptive {TB} had {M}. tuberculosis-positive results for nasogastric aspiration ({NGA}) samples, and 7 (77.8%) of those children also had {M}. tuberculosis-positive {X}pert results for stool samples. {A}dditionally, {M}. tuberculosis was detected in the stool samples but not the {NGA} samples from 2 children. {T}he {SOS} stool processing method makes use of the standard {X}pert assay kit, without the need for additional supplies or equipment. {T}he method can potentially be rolled out to any {X}pert site, bringing a bacteriologically confirmed diagnosis of {TB} in children closer to the point of care.},
  keywords = {tuberculosis ; children ; stool ; diagnosis ; {E}thiopia ; simple one-step ; stool method ; {X}pert {MTB}/{RIF} ; bacteriologically confirmed ; point of care ; sputum ; {ETHIOPIE}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {59},
  numero = {8},
  pages = {e00406--21 [11 ]},
  ISSN = {0095-1137},
  year = {2021},
  DOI = {10.1128/jcm.00406-21},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083293},
}