Polanco F. A., Martinezguerra M. M., Marques V., Villa-Navarro F., Perez G. H. B., Cheutin M. C., Dejean T., Hocdé Régis, Juhel J. B., Maire E., Manel S., Spescha M., Valentini A., Mouillot D., Albouy C., Pellissier L. (2021). Detecting aquatic and terrestrial biodiversity in a tropical estuary using environmental DNA. Biotropica, 53 (6), 1606-1619. ISSN 0006-3606.
Titre du document
Detecting aquatic and terrestrial biodiversity in a tropical estuary using environmental DNA
Année de publication
2021
Auteurs
Polanco F. A., Martinezguerra M. M., Marques V., Villa-Navarro F., Perez G. H. B., Cheutin M. C., Dejean T., Hocdé Régis, Juhel J. B., Maire E., Manel S., Spescha M., Valentini A., Mouillot D., Albouy C., Pellissier L.
Source
Biotropica, 2021,
53 (6), 1606-1619 ISSN 0006-3606
Estuaries are characterized by a tidal regime and are strongly influenced by hydrodynamics and host diverse and highly dynamic habitats, from fresh, brackish, or saltwater to terrestrial, whose biodiversity is especially difficult to monitor. Here, we investigated the potential of environmental DNA (eDNA) metabarcoding, with three primer sets targeting different regions of the mitochondrial DNA 12S ribosomal RNA gene, to detect vertebrate diversity in the estuary of the Don Diego River in Colombia. With eDNA, we detected not only aquatic organisms, including fishes, amphibians, and reptiles, but also a large diversity of terrestrial, arboreal, and flying vertebrates, including mammals and birds, living in the estuary surroundings. Further, the eDNA signal remained relatively localized along the watercourse. A transect from the deep outer section of the estuary, across the river mouth toward the inner section of the river, showed marked taxonomic turnover from typical marine to freshwater fishes, while eDNA of terrestrial and arboreal species was mainly found in the inner section of the estuary. Our results indicate that eDNA enables the detection of a large diversity of vertebrates and could become an important tool for biodiversity monitoring in estuaries, where water integrates information across the ecosystem. Abstract in Spanish is available with online material.
Plan de classement
Limnologie biologique / Océanographie biologique [034]
;
Ecologie, systèmes aquatiques [036]
;
Sciences du monde animal [080]
;
Etudes, transformation, conservation du milieu naturel [082]
Description Géographique
COLOMBIE ; ZONE TROPICALE
Localisation
Fonds IRD [F B010082664]
Identifiant IRD
fdi:010082664
