@article{fdi:010082664, title = {{D}etecting aquatic and terrestrial biodiversity in a tropical estuary using environmental {DNA}}, author = {{P}olanco, {F}. {A}. and {M}artinezguerra, {M}. {M}. and {M}arques, {V}. and {V}illa-{N}avarro, {F}. and {P}erez, {G}. {H}. {B}. and {C}heutin, {M}. {C}. and {D}ejean, {T}. and {H}ocd{\'e}, {R}{\'e}gis and {J}uhel, {J}. {B}. and {M}aire, {E}. and {M}anel, {S}. and {S}pescha, {M}. and {V}alentini, {A}. and {M}ouillot, {D}. and {A}lbouy, {C}. and {P}ellissier, {L}.}, editor = {}, language = {{ENG}}, abstract = {{E}stuaries are characterized by a tidal regime and are strongly influenced by hydrodynamics and host diverse and highly dynamic habitats, from fresh, brackish, or saltwater to terrestrial, whose biodiversity is especially difficult to monitor. {H}ere, we investigated the potential of environmental {DNA} (e{DNA}) metabarcoding, with three primer sets targeting different regions of the mitochondrial {DNA} 12{S} ribosomal {RNA} gene, to detect vertebrate diversity in the estuary of the {D}on {D}iego {R}iver in {C}olombia. {W}ith e{DNA}, we detected not only aquatic organisms, including fishes, amphibians, and reptiles, but also a large diversity of terrestrial, arboreal, and flying vertebrates, including mammals and birds, living in the estuary surroundings. {F}urther, the e{DNA} signal remained relatively localized along the watercourse. {A} transect from the deep outer section of the estuary, across the river mouth toward the inner section of the river, showed marked taxonomic turnover from typical marine to freshwater fishes, while e{DNA} of terrestrial and arboreal species was mainly found in the inner section of the estuary. {O}ur results indicate that e{DNA} enables the detection of a large diversity of vertebrates and could become an important tool for biodiversity monitoring in estuaries, where water integrates information across the ecosystem. {A}bstract in {S}panish is available with online material.}, keywords = {biodiversity ; biomonitoring ; {C}aribbean {S}ea ; {C}olombia ; {D}on {D}iego {R}iver ; environmental {DNA} ; {S}ierra {N}evada de {S}anta {M}arta ; tropical ecosystem ; vertebrate ; {COLOMBIE} ; {ZONE} {TROPICALE} ; {SIERRA} {NEVADA} {DE} {SANTA} {MARTA} ; {CARAIBES} {MER} ; {DON} {DIEGO} {COURS} {D}'{EAU}}, booktitle = {}, journal = {{B}iotropica}, volume = {53}, numero = {6}, pages = {1606--1619}, ISSN = {0006-3606}, year = {2021}, DOI = {10.1111/btp.13009}, URL = {https://www.documentation.ird.fr/hor/fdi:010082664}, }