Raharimalala N., Rombauts S., McCarthy A., Garavito A., Orozco-Arias S., Bellanger L., Morales-Correa A. Y., Froger S., Michaux S., Berry V., Metairon S., Fournier C., Lepelley M., Mueller L., Couturon Emmanuel, Hamon Perla, Rakotomalala J. J., Descombes P., Guyot Romain, Crouzillat D. (2021). The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago. Scientific Reports - Nature, 11 (1), p. 8119 [14 p.]. ISSN 2045-2322.
Titre du document
The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
Année de publication
2021
Auteurs
Raharimalala N., Rombauts S., McCarthy A., Garavito A., Orozco-Arias S., Bellanger L., Morales-Correa A. Y., Froger S., Michaux S., Berry V., Metairon S., Fournier C., Lepelley M., Mueller L., Couturon Emmanuel, Hamon Perla, Rakotomalala J. J., Descombes P., Guyot Romain, Crouzillat D.
Source
Scientific Reports - Nature, 2021,
11 (1), p. 8119 [14 p.] ISSN 2045-2322
Caffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the Comoro archipelago. We predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. Comparative analyses with the African Robusta coffee genome (C. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the Coffea genus. In this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. These findings pave the way for further characterization of caffeine-free species in the Coffea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
Description Géographique
COMORES
Localisation
Fonds IRD [F B010081437]
Identifiant IRD
fdi:010081437
