@article{fdi:010081437,
  title = {{T}he absence of the caffeine synthase gene is involved in the naturally decaffeinated status of {C}offea humblotiana, a wild species from {C}omoro archipelago},
  author = {{R}aharimalala, {N}. and {R}ombauts, {S}. and {M}c{C}arthy, {A}. and {G}aravito, {A}. and {O}rozco-{A}rias, {S}. and {B}ellanger, {L}. and {M}orales-{C}orrea, {A}. {Y}. and {F}roger, {S}. and {M}ichaux, {S}. and {B}erry, {V}. and {M}etairon, {S}. and {F}ournier, {C}. and {L}epelley, {M}. and {M}ueller, {L}. and {C}outuron, {E}mmanuel and {H}amon, {P}erla and {R}akotomalala, {J}. {J}. and {D}escombes, {P}. and {G}uyot, {R}omain and {C}rouzillat, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}affeine is the most consumed alkaloid stimulant in the world. {I}t is synthesized through the activity of three known {N}-methyltransferase proteins. {H}ere we are reporting on the 422-{M}b chromosome-level assembly of the {C}offea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the {C}omoro archipelago. {W}e predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. {C}omparative analyses with the {A}frican {R}obusta coffee genome ({C}. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the {C}offea genus. {I}n this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. {T}hese findings pave the way for further characterization of caffeine-free species in the {C}offea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.},
  keywords = {{COMORES}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {11},
  numero = {1},
  pages = {8119 [14 p.]},
  ISSN = {2045-2322},
  year = {2021},
  DOI = {10.1038/s41598-021-87419-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081437},
}