Publications des scientifiques de l'IRD

Compaore C. F. A., Ilboudo H., Kabore J., Kabore J. W., Camara O., Bamba M., Sakande H., Kone M., Camara M., Kaba D., Belem A. M. G., Deborggraeve S., Buscher P., Bucheton Bruno, Lejon Veerle, Jamonneau Vincent. (2020). Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination. Experimental Parasitology, 219, p. 108014 [6 p.]. ISSN 0014-4894.

Titre du document
Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000591769600011
Auteurs
Compaore C. F. A., Ilboudo H., Kabore J., Kabore J. W., Camara O., Bamba M., Sakande H., Kone M., Camara M., Kaba D., Belem A. M. G., Deborggraeve S., Buscher P., Bucheton Bruno, Lejon Veerle, Jamonneau Vincent
Source
Experimental Parasitology, 2020, 219, p. 108014 [6 p.] ISSN 0014-4894
The objective set by WHO to reach elimination of human African trypanosomiasis (HAT) as a public health problem by 2020 is being achieved. The next target is the interruption of gambiense-HAT transmission in humans by 2030. To monitor progress towards this target, in areas where specialized local HAT control capacities will disappear, is a major challenge. Test specimens should be easily collectable and safely transportable such as dried blood spots (DBS). Monitoring tests performed in regional reference centres should be reliable, cheap and allow analysis of large numbers of specimens in a high-throughput format. The aim of this study was to assess the analytical sensitivity of Loopamp, M18S quantitative real-time PCR (M18S qPCR) and TgsGP qPCR as molecular diagnostic tests for the presence of Trypanosoma brucei gambiense in DBS. The sensitivity of the Loopamp test, with a detection limit of 100 trypanosomes/mL, was in the range of parasitaemias commonly observed in HAT patients, while detection limits for M18S and TgsGP qPCR were respectively 1000 and 10,000 trypanosomes/mL. None of the tests was entirely suitable for high-throughput use and further development and implementation of sensitive high-throughput molecular tools for monitoring HAT elimination are needed.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
AFRIQUE SUBSAHARIENNE
Localisation
Fonds IRD [F B010080419]
Identifiant IRD
fdi:010080419
Contact