@article{fdi:010080419,
  title = {{A}nalytical sensitivity of loopamp and quantitative real-time {PCR} on dried blood spots and their potential role in monitoring human {A}frican trypanosomiasis elimination},
  author = {{C}ompaore, {C}. {F}. {A}. and {I}lboudo, {H}. and {K}abore, {J}. and {K}abore, {J}. {W}. and {C}amara, {O}. and {B}amba, {M}. and {S}akande, {H}. and {K}one, {M}. and {C}amara, {M}. and {K}aba, {D}. and {B}elem, {A}. {M}. {G}. and {D}eborggraeve, {S}. and {B}uscher, {P}. and {B}ucheton, {B}runo and {L}ejon, {V}eerle and {J}amonneau, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he objective set by {WHO} to reach elimination of human {A}frican trypanosomiasis ({HAT}) as a public health problem by 2020 is being achieved. {T}he next target is the interruption of gambiense-{HAT} transmission in humans by 2030. {T}o monitor progress towards this target, in areas where specialized local {HAT} control capacities will disappear, is a major challenge. {T}est specimens should be easily collectable and safely transportable such as dried blood spots ({DBS}). {M}onitoring tests performed in regional reference centres should be reliable, cheap and allow analysis of large numbers of specimens in a high-throughput format. {T}he aim of this study was to assess the analytical sensitivity of {L}oopamp, {M}18{S} quantitative real-time {PCR} ({M}18{S} q{PCR}) and {T}gs{GP} q{PCR} as molecular diagnostic tests for the presence of {T}rypanosoma brucei gambiense in {DBS}. {T}he sensitivity of the {L}oopamp test, with a detection limit of 100 trypanosomes/m{L}, was in the range of parasitaemias commonly observed in {HAT} patients, while detection limits for {M}18{S} and {T}gs{GP} q{PCR} were respectively 1000 and 10,000 trypanosomes/m{L}. {N}one of the tests was entirely suitable for high-throughput use and further development and implementation of sensitive high-throughput molecular tools for monitoring {HAT} elimination are needed.},
  keywords = {{T}rypanosoma brucei gambiense ; {D}iagnosis ; {D}ried blood spots ; {L}oopamp ; {Q}uantitative real-time {PCR} ; {S}ensitivity ; {F}easibility ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {219},
  numero = {},
  pages = {108014 [6 p.]},
  ISSN = {0014-4894},
  year = {2020},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080419},
}